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Study Information
Study Attribute Value
CEBS Accession Number: 002-02755-0005-0000-6
Chemical Name: Pyrene
CASRN: 129-00-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 398036
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 105 107 52 16 21
    107 117 63 20
    92 81 84 30
    105 96 59 19
Test Chemical Pyrene 
7.8       
 91 72 68 25 23
    86 79 56 22
  
15.6       
 85 82 74 29 21
    101 97 39 13
  
31.3       
 91r 79 56 21
    127r 93 66 17
  
62.5       
 77 77 30 13 18
    57 57 40 23
  
125         
 66 46 52 26 23
    72# 66 40.5 19
  
250         
 91 49 68 25 26
    77 44 62 27
Positive Control Ethyl Methane Sulfonate 
500         
 17 7 449 872 813*
    25# 12 606 824
    26 7 584 744

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 115 120 58 17 19
    95 108 53 19
    102 94 62 20
    98 78 63 21
Test Chemical Pyrene 
7.8       
 102 102 60 20 19
    105 124 57 18
    100 110 58 19
  
15.6       
 97 70 60 21 20
    82 90 39 16
    104 81 76 24
  
31.3       
 81# 82 57 24 22
    87 86 58 22
    104 81 65 21
  
62.5       
 87 82 46 18 21
    93 92 66 24
    101 87 67 22
  
125         
 92 10 98 35 30
    93 48 73 26
    90 50 80 30
Positive Control Ethyl Methane Sulfonate 
500         
 36# 15 861 794 811*
    34 13 919 892
    41 14 908 747

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 110 100 34 23
    98 82 60 20
    86 99 53 21
    86 108 47 18
Test Chemical Pyrene 
 0.24      
 76 86 100 44 44*
    88 86 118 45
  
 0.49      
 101 123 97 32 33
    96# 110 94.5 33
  
 0.98      
 75 85 83 37 38*
    109 85 130 40
  
1.95      
 70 68 60 28 36
    95 80 125 44
  
3.9       
 60 37 110 61 53*
    78 51 104 44
  
7.8       
 56 10 227 136 123*
    69 14 227 110
Positive Control 3-Methylcholanthrene 
5         
 59 37 445 252 308*
    45 21 492 369
    55 22 499 303

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 86 67 27 24
    95 125 61 21
    97 94 72 25
    81 95 56 23
Test Chemical Pyrene 
 0.25      
 80 129 72 30 32
    64 80 64 34
  
 0.5       
 72 84 75 35 39*
    77 110 102 44
  
1         
 79 75 110 46 45*
    86 83 115 44
  
2         
 99 67 101 34 38
    94 88 118 42
  
4         
 88 49 160 61 55*
    71 37 107 50
  
6         
 77 24 108 47 39
    91 43 86 31
Positive Control 3-Methylcholanthrene 
5         
 54 36 388 241 215*
    59 41 371 208
    59 36 343 194

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 64# 96 73 38 47
    87 154 127 49
    52 51 82 52
    69 100 102 49
Test Chemical Pyrene 
1         
 58 81 100 58 60
    69 66 131 63
  
2         
 49 52 138 93 80*
    60 67 120 67
  
4         
 65# 48 138 71 99*
    54 44 206 127
  
5         
 71 20 383 179 104*
    80# 58 184.5 77
    72 62 123 57
  
6         
 65# 35 178.5 91 99*
    65 29 189 97
    65 12 212 108
  
7         
 65 39 214 110 121*
    74 17 302 137
    50 24 175 116
  
8         
 57 13 278 164 166*
    51 12 260 170
    51 11 251 165
Positive Control 3-Methylcholanthrene 
5         
 34 3 340 332 347*
    15# 1 205.5 447
    21 2 162 261

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination