# 1. Gene Aliases

Curators will manually extract data from “Aliases for xx Gene” section of the GeneCards database, i.e., https://www.genecards.org/cgi-bin/carddisp.pl?gene=HAVCR1.

# 2. Association with Toxicity and/or Disease at a Transcriptional Level

Curators will manual exact data from publications relating to gene expression in tissue of interest. Note that only include publications related to transcriptional level associations. In vitro models can be used as second tier evidence as long as the cells used are primary cells from rat. We do not regard mutagenicity as an adverse effect unless the study is focusing on model of action or association mechanisms. Studies that show the gene of interest association with disease or toxicity using IHC or ELISA (or other protein assays) should not be included here (Purely on mRNA). If no information relating to this topic found, curators can put “No relevant information pertaining to this subject matter was identified in the existing body of literature."

# 3. Summary of Protein Family and Structure

Curators will need to provide basic information about the protein, including the AA length, protein domains, and extract information on how domains are related to the protein function. Curators can find relate information under “GeneName INFORMATION” section and “PROTEIN FUNCTION” section of HPA (i.e, https://www.proteinatlas.org/ENSG00000113249-HAVCR1), “Proteins for Gene” section of Genecards (i.e., https://www.genecards.org/cgi-bin/carddisp.pl?gene=HAVCR1) and “Function” section from Uniprot by the protein, i.e.,: https://www.uniprot.org/uniprotkb/Q96D42/entry). Relationship of the protein domains with the protein functions could also be extracted based on publications.

- Protein Accession:
- Size:
- Molecular mass:
- Domains:
- Blocks:
- Family:

# 6. GO Terms, MSigDB Signatures, Pathways Containing Gene with Descriptions of Gene Sets

## Pathways:

Curators can refer to the Genecards database-> “Pathways & Interactions for Gene” section. Emphasis was placed on Reactome database pathways within GeneCards, with a focus on granular pathway terms. High-level pathway branches were excluded in favor of more specific terms.When Reactome annotations were unavailable in GeneCards, pathways were sourced from publications, and their descriptions were obtained from diverse sources, including WikiPathways (https://www.wikipathways.org/), KEGG (https://www.genome.jp/kegg/pathway.html), and Review papers.

# 9. Mechanistic Information

(Why Does Expression Change with Toxicity and/or Disease?) – Curator will manually extract info for this section based on publications. This section should be written in a narrative style. If necessary, results from several publications should be summarized. If no information relating to this topic found, curators can put “No relevant information pertaining to this subject matter was identified in the existing body of literature."

# 10. Upstream Regulators

Curators will extract data for this section based on relevant publications. If no information relating to this topic found, curators can put “No relevant information pertaining to this subject matter was identified in the existing body of literature."

# 12. Role of Gene in Other Tissues

Curators will extract data for this section based on relevant publications. If no information relating to this topic found, curators can put “No relevant information pertaining to this subject matter was identified in the existing body of literature."