
-   Downregulation of OY-TES-1 by RNAi resulted in significant changes in expression of NANOG, CD9, CCND2 and CDCA3 in the liver cancer cell line BEL-7404. NANOG, CD9, CCND2 and CDCA3 may be involved in cell proliferation, migration, invasion and apoptosis, yet also may be functionally related to each other and OY-TES-1 [PMID: 25673160].
-   HomeoboxB3 (HoxB3) mRNA and protein are overexpressed in primary prostate cancer tissues compared to the adjacent normal prostate tissues and its overexpression is associated with higher Gleason grade clinical stage. Kaplan and Meier analysis showed that HoxB3 overexpression predicts poor survival outcome in prostate cancer. Overexpression of HoxB3 was also shown to promote LNCaP cell proliferation and migration in vitro. Depletion of HoxB3 in PC-3 cells decreased the capacity of proliferation in a cell division cycle associated 3 (CDCA3)-dependent manner both in vitro and in vivo. ChIP analysis indicated that HoxB3 can bind to the CDCA3 promoter region and transactivate the CDCA3 expression [PMID: 23219899].
-   MYBL2 was elevated in bladder cancer (BLCA) tissues and significantly correlated with clinicopathological parameters and cancer-specific survival in BLCA patients. Phenotypic assays showed that MYBL2 deficiency suppressed the proliferation and migration of BLCA cells in vitro and in vivo, whereas MYBL2 overexpression contributed to the opposite phenotype. Mechanistically, MYBL2 could bind to the promoter of its downstream target gene cell division cycle-associated protein 3 (CDCA3) and transactivate it, which in turn promoted the malignant phenotype of BLCA cells. Further investigations revealed that MYBL2 interacted with forkhead box M1 (FOXM1) to co-regulate the transcription of CDCA3 [PMID: 36071275].
-   CDH1: In non-small cell lung cancer (NSCLC) cells, CDCA3 protein levels are regulated by the ubiquitin ligase APC/C and cofactor Cdh1. Results showed that the degradation of CDCA3 is modulated by activity of casein kinase 2 (CK2) which promotes an interaction between CDCA3 and Cdh1 [PMID: 34050247].
