
-   A Direct Repeat 1 (DR1) located in Acot1 promoter in mouse, bound PPARalpha/retinoid X receptor alpha (RXRalpha) and HNF4alpha as demonstrated by ChiP assay; whereas the binding in ChIP was abrogated in the PPARalpha and HNF4alpha knockout mouse models. Reporter gene assays showed activation of the Acot1 promoter in cells by the PPARalpha agonist Wy-14,643 after cotransfection with PPARalpha/RXRalpha. However, transfection with a plasmid containing HNF4alpha also resulted in an increase in promoter activity. Together, these data show that Acot1 is under regulation by an interplay between HNF4alpha and PPARalpha [PMID: 17485727].
-   Upregulation of Acot1 was observed in response to fasting and clofibrate treatment in livers of mice [PMID: 9490035].
-   Mouse study showed that in the skeletal muscle, *Acot1* was found to be up-regulated by fasting, ketogenic diet, and high-fat diet [PMID: 25760036].
