BISMA results:
Reference sequence (original) : CTTCCCAAAGTGCTAGGATTATAGGCGTGAGCCTCCGCACCTGGCATGCCTGGCCATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATCCACGAAAGTAAAGTGCCTAGGGCCCATCAATGTCAGATGCAGCCACGTGGACAAAGAACACTCCCTGAGCA
Reference sequence (converted): TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA
Number CpG in Reference sequence: 4
Parameters used:
Alignment mode: pairwise alignment of reference sequence and experimental sequences
Lower threshold conversion rate: 95%
Lower threshold sequence identity: 90%
Upper threshold of N-sites at cytosine positions: 20%
Upper threshold insertions/deletions: 20%
Final sequence alignment of the sequences included:
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1 1 1 1 |
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2 4 6 8 0 2 4 6 |
Number |
Sequence_id |
0 0 0 0 0 0 0 0 |
|
Reference |
TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA |
1 | 26KP-Hyal11N5bselection | TTTTTTAAAGTGTTAGGATTATAGGTGTGAGTTTTTGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTT-AAGGAGGAAAAGATTTATGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTATGTGGATAAAGAATATTTTTTGAGTA |
2 | 91KP-Hyal11N1selection | TTTTTTAAAGTGTTAGGATTATAGGTGTGAGTTTTTGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTT-AAGGAGGAAAAGATTTATGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTATGTGGATAAAGAATATTTTTTGAGTA |
3 | 92KP-Hyal11N2selection | TTTTTTAAAGTGTTAGGATTATAGGTGTGAGTTTTTGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTT-AAGGAGGAAAAGATTTATGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTATGTGGATAAAGAATATTTTTTGAGTA |
4 | 93KP-Hyal11N3selection | TTTTTTAAAGTGTTAGGATTATAGGTGTGAGTTTTTGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTT-AAGGAGGAAAAGATTTATGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTATGTGGATAAAGAATATTTTTTGAGTA |
5 | 94KP-Hyal11N4selection | TTTTTTAAAGTGTTAGGATTATAGGTGTGAGTTTTTGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTT-AAGGAGGAAAAGATTTATGAAAGTAAAGTGTTTAGGGTTCATTAATGTTAGATGTAGTTATGTGGATAAAGAATATTTTTTGAGTA |
6 | 96KP-Hyal11N6selection | TTTTTTAAAGTGTTAGGATTATAGGTGTGAGTTTTTGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTT-AAGGAGGAAAAGATTTATGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTATGTGGATAAAGAATATTTTTTGAGTA |
Legend:
methylated CpG position
unmethylated CpG position
unconverted cytosine
cytosine at unexpected position