BISMA results:
Reference sequence (original) : CTTCCCAAAGTGCTAGGATTATAGGCGTGAGCCTCCGCACCTGGCATGCCTGGCCATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATCCACGAAAGTAAAGTGCCTAGGGCCCATCAATGTCAGATGCAGCCACGTGGACAAAGAACACTCCCTGAGCA
Reference sequence (converted): TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA
Number CpG in Reference sequence: 4
Parameters used:
Alignment mode: pairwise alignment of reference sequence and experimental sequences
Lower threshold conversion rate: 95%
Lower threshold sequence identity: 90%
Upper threshold of N-sites at cytosine positions: 20%
Upper threshold insertions/deletions: 20%
Final sequence alignment of the sequences included:
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1 1 1 1 |
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2 4 6 8 0 2 4 6 |
Number |
Sequence_id |
0 0 0 0 0 0 0 0 |
|
Reference |
TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA |
1 | 49KP-Hyal11R1selection | TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA |
2 | 50KP-Hyal11R2selection | TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA |
3 | 51KP-Hyal11R3selection | TTTTTTAAAGTGTTAGGATTATAGGCGTGAGTTTTCGTATTTGGTATGTTTGGTTATATTTTAAAATATTTTTTTTAAGGAGGAAAAGATTTACGAAAGTAAAGTGTTTAGGGTTTATTAATGTTAGATGTAGTTACGTGGATAAAGAATATTTTTTGAGTA |
Legend:
methylated CpG position
unmethylated CpG position
unconverted cytosine
cytosine at unexpected position