NIEHS Report 13: NIEHS Report on the In Vivo Repeat Dose Biological Potency Study of 1,4-Dichlorobenzene (CASRN 106-46-7) in Sprague Dawley (Hsd:Sprague Dawley® SD®) Rats and B6D2F1/Crl Mice via Whole Body Respiratory Exposure

Short-Term

Materials and Methods

• Materials and Methods (356 KB)

Theoretical Inhaled Dose

• Theoretical Inhaled Dose (170 KB)

Apical Benchmark Dose Analysis - Rats

• BMD, NOEL and LOEL Summary for Apical Endpoints (173 KB)
• BMDExpress Project File Rat Apical (bm2 format) (134 KB)
• Clinical Chemistry Summary (120 KB)
• Hematology Summary (49 KB)
• Mean Body Weight Summary (85 KB)
• Organ Weights Summary (77 KB)

Genomic Benchmark Dose Analysis - Rats

GEO/SRA: https://www.ncbi.nlm.nih.gov/bioproject/1304578

• All Organ Principal Components Analysis Files (1 MB)
• Animal and Fastaq Metadata (925 KB)
• Attenuation-adjusted Raw Read Counts Rat Study (3 MB)
• Benchmark Dose Analysis Rat Heart (240 KB)
• Benchmark Dose Analysis Rat Kidney (210 KB)
• Benchmark Dose Analysis Rat Liver (1 MB)
• Benchmark Dose Analysis Rat Lung (509 KB)
• Benchmark Dose Analysis Rat Ovary (383 KB)
• BMDExpress Expression Data Heart (1 MB)
• BMDExpress Expression Data Kidney (1 MB)
• BMDExpress Expression Data Liver (1 MB)
• BMDExpress Expression Data Lung (1 MB)
• BMDExpress Expression Data Ovary (1 MB)
• BMDExpress GO Biological Process BMD Results Rat Heart (149 KB)
• BMDExpress GO Biological Process BMD Results Rat Kidney (313 KB)
• BMDExpress GO Biological Process BMD Results Rat Liver (3 MB)
• BMDExpress GO Biological Process BMD Results Rat Lung (1 MB)
• BMDExpress GO Biological Process BMD Results Rat Ovary (1 MB)
• BMDExpress Individual Gene BMD Results Rat Heart (82 KB)
• BMDExpress Individual Gene BMD Results Rat Kidney (82 KB)
• BMDExpress Individual Gene BMD Results Rat Liver (363 KB)
• BMDExpress Individual Gene BMD Results Rat Lung (145 KB)
• BMDExpress Individual Gene BMD Results Rat Ovary (119 KB)
• BMDExpress Project File Rat Genomic (bm2 format) (9 MB)
• BMDExpress Project File Rat Genomic (JSON format) (59 MB)
• Curve Fit Rat Heart (20 KB)
• Curve Fit Rat Kidney (17 KB)
• Curve Fit Rat Liver (83 KB)
• Curve Fit Rat Lung (41 KB)
• Curve Fit Rat Ovary (31 KB)
• Most Potent GO Biological Process In Each Tissue (49 KB)
• One-way ANOVA Rat Heart (11 KB)
• One-way ANOVA Rat Kidney (9 KB)
• One-way ANOVA Rat Liver (103 KB)
• One-way ANOVA Rat Lung (11 KB)
• One-way ANOVA Rat Ovary (17 KB)
• Rat DCB eFDR Synthetic Null Analysis (33 MB)
• TempO-Seq S1500v1.2 Full Manifest Rattus Norvegicus (259 KB)
• Top 10 Genes Ranked by Potency of Perturbation Heart (258 KB)
• Top 10 Genes Ranked by Potency of Perturbation Kidney (287 KB)
• Top 10 Genes Ranked by Potency of Perturbation Liver (249 KB)
• Top 10 Genes Ranked by Potency of Perturbation Lung (198 KB)
• Top 10 Genes Ranked by Potency of Perturbation Ovary (258 KB)
• Top 10 GO Biological Process Gene Sets Heart (146 KB)
• Top 10 GO Biological Process Gene Sets Kidney (149 KB)
• Top 10 GO Biological Process Gene Sets Liver (133 KB)
• Top 10 GO Biological Process Gene Sets Lung (147 KB)
• Top 10 GO Biological Process Gene Sets Ovary (145 KB)
• Transcriptomics Data QC Report (4 MB)

Study Tables - Rats

• I01 - Animal Removal Summary (43 KB)
• I02 - Animal Removals (216 KB)
• I04 - Mean Body Weights and Survival (80 KB)
• I04G - Mean Body Weight Gain (49 KB)
• I05 - Clinical Observations Summary (90 KB)
• PA06 - Organ Weights Summary (63 KB)
• PA41 - Clinical Chemistry Summary (150 KB)
• PA43 - Hematology Summary (75 KB)
• PA48 - Blood and Tissue Concentration (34 KB)

Individual Animal Data - Rats

• Individual Animal Body Weight Data (14 KB)
• Individual Animal Clinical Chemistry Data (15 KB)
• Individual Animal Clinical Observations Data (12 KB)
• Individual Animal Hematology Data (18 KB)
• Individual Animal Organ Weight Data (7 KB)
• Individual Animal Removal Reasons Data (6 KB)

Apical Benchmark Dose Analysis - Mice

• BMD, NOEL and LOEL Summary for Apical Endpoints (93 KB)
• BMDExpress Project File Mouse Apical (bm2 format) (134 KB)
• Clinical Chemistry Summary (80 KB)
• Mean Body Weight Summary (78 KB)
• Organ Weights Summary (92 KB)

Genomic Benchmark Dose Analysis - Mice

GEO/SRA: https://www.ncbi.nlm.nih.gov/bioproject/1304935

• All Organ Principal Components Analysis Files (909 KB)
• Animal and Fastaq Metadata (120 KB)
• Attenuation-adjusted Raw Read Counts Mouse Study (3 MB)
• Benchmark Dose Analysis Mouse Heart (93 KB)
• Benchmark Dose Analysis Mouse Kidney (180 KB)
• Benchmark Dose Analysis Mouse Liver (2 MB)
• Benchmark Dose Analysis Mouse Lung (155 KB)
• Benchmark Dose Analysis Mouse Ovary (49 KB)
• BMDExpress Expression Data Heart (1 MB)
• BMDExpress Expression Data Kidney (1 MB)
• BMDExpress Expression Data Liver (1 MB)
• BMDExpress Expression Data Lung (1 MB)
• BMDExpress Expression Data Ovary (1 MB)
• BMDExpress GO Biological Process BMD Results Mouse Heart (12 KB)
• BMDExpress GO Biological Process BMD Results Mouse Kidney (33 KB)
• BMDExpress GO Biological Process BMD Results Mouse Liver (5 MB)
• BMDExpress GO Biological Process BMD Results Mouse Lung (41 KB)
• BMDExpress GO Biological Process BMD Results Mouse Ovary (5 KB)
• BMDExpress Individual Gene BMD Results Mouse Heart (31 KB)
• BMDExpress Individual Gene BMD Results Mouse Kidney (41 KB)
• BMDExpress Individual Gene BMD Results Mouse Liver (584 KB)
• BMDExpress Individual Gene BMD Results Mouse Lung (53 KB)
• BMDExpress Individual Gene BMD Results Mouse Ovary (14 KB)
• BMDExpress Project File Mouse Genomic (bm2 format) (9 MB)
• BMDExpress Project File Mouse Genomic (JSON format) (53 MB)
• Curve Fit Mouse Heart (8 KB)
• Curve Fit Mouse Kidney (12 KB)
• Curve Fit Mouse Liver (136 KB)
• Curve Fit Mouse Lung (13 KB)
• Curve Fit Mouse Ovary (5 KB)
• Most Potent GO Biological Process In Each Tissue (39 KB)
• Mouse DCB eFDR Synthetic Null Analysis (35 MB)
• One-way ANOVA Mouse Heart (3 KB)
• One-way ANOVA Mouse Kidney (4 KB)
• One-way ANOVA Mouse Liver (173 KB)
• One-way ANOVA Mouse Lung (11 KB)
• One-way ANOVA Mouse Ovary (558 B)
• TempO-Seq S1500v1.2 Full Manifest Mus Musculus (355 KB)
• Top 10 Genes Ranked by Potency of Perturbation Heart (446 KB)
• Top 10 Genes Ranked by Potency of Perturbation Kidney (386 KB)
• Top 10 Genes Ranked by Potency of Perturbation Liver (364 KB)
• Top 10 Genes Ranked by Potency of Perturbation Lung (341 KB)
• Top 10 Genes Ranked by Potency of Perturbation Ovary (273 KB)
• Top 10 GO Biological Process Gene Sets Heart (93 KB)
• Top 10 GO Biological Process Gene Sets Kidney (78 KB)
• Top 10 GO Biological Process Gene Sets Liver (78 KB)
• Top 10 GO Biological Process Gene Sets Lung (76 KB)
• Top 10 GO Biological Process Gene Sets Ovary (107 KB)
• Transcriptomics Data QC Report (4 MB)

Study Tables - Mice

• I01 - Animal Removal Summary (41 KB)
• I02 - Animal Removals (191 KB)
• I04 - Mean Body Weights and Survival (73 KB)
• I04G - Mean Body Weight Gain (43 KB)
• I05 - Clinical Observations Summary (43 KB)
• PA06 - Organ Weights Summary (59 KB)
• PA41 - Clinical Chemistry Summary (138 KB)
• PA48 - Blood and Tissue Concentration (34 KB)

Individual Animal Data - Mice

• Individual Animal Body Weight Data (12 KB)
• Individual Animal Clinical Chemistry Data (14 KB)
• Individual Animal Clinical Observations Data (7 KB)
• Individual Animal Organ Weight Data (6 KB)
• Individual Animal Removal Reasons Data (6 KB)

Analysis Pipeline Evaluation

• Analysis Pipeline Evaluation (90 MB)
• DTT tBMD Pipeline bm2 (73 MB)
• EPA tBMD Pipeline bm2 (62 MB)

BMDExpress Preview Software

BMDExpress Software: https://cebs-ext.niehs.nih.gov/cahs/file/download/2025_5Day_BMD_TM/BMDExpress_PreviewSoftware_08152025.exe